Ashley Gravelle, 35, of Port Dover died March 2 in an opioid-related death.
Morgan Daniel Fick, 21, was charged on March 14 with manslaughter, possession of fentanyl for the purposes of trafficking.
On March 30th Carilyn Michelle Deming, 23, of Norfolk County was charged with manslaughter.
Const. Joe Varga, head of the Norfolk OPP, said police services across Canada continue to investigate opioid-related deaths with an eye to laying criminal charges.